Ion Exchange Chromatography (IEX)

Ion Exchange chromatography is a binding technique with very high binding capacities, high flow characteristics and potentially excellent resolution. It is therefore perfect for the separation of large volumes of sample (fluid feed)
and fits well into the early or capture step of a purification methodology.

In an aqueous environment most biomolecules are charged due to the presence of ionic groups within their structure. Depending on the pH of the environment these biomolecules carry a net positive charge, a net negative charge or no net xharge. The pH value at which a biomolecule carries no net charge is called its isoelectric point (pI). The pI is highly specific for each type of biomolecule. When exposed to a pH below its pI a biomolecule will carry a positive charge.